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Analysis:

  • 1-hydroxy-pyrene in urine
  • 5-Nitro-gamma tocopherol in plasma
  • Acids (citrate cycle analytes): lactic acid, pyruvic acid, alpha-ketoglutaric acid, from liver tissue
  • Aldehydes (formaldehyde, acetaldehyde, acrolein) by LCMS
  • Alkaloids from urine, saliva, hair, plants by LCMS (see nicotine, cotinine, Areca alkaloids)
  • Alkylresercinols from serum
  • Ammonia in urine
  • APO-A1 in plasma by AA
  • Apo-B in plasma by AA
  • Apo-E in plasma by AA
  • Areca (Betel) alkaloids from urine, saliva, plants by LCMS (arecoline, arecaidine, guvacine, guvacoline)
  • Bisphenol-A in urine by LC/MSMS
  • Caffeine and its metabolites in urine
  • Calcium in plasma by AA
  • Carotenoids including their isomers in plasma by HPLC/PDA (luteins, zeaxanthins, alpha- and beta-cryptoxanthins, lycopenes, alpha- and beta-carotenes)
  • Chavibetol from urine, saliva, plants by LCMS
  • Cholesterol (total) in plasma by AA
  • Cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, ursodeoxycholic acid, and hyodeoxycholid acid from serum
  • Choline, betaine, carnitine, trimethylamine-N-oxide from plasma or serum (TMAO) by LCMS
  • Choline, betaine, CDP-choline, phosphocholine, glycerophosphocholine from liver tissue
  • Citric acid, and the most abundant fatty acids from liver tissue
  • Coenzyme Q10 (ubiquinol-10 and ubiquinone-10) by HPLC/PDA
  • Cotinine and hydroxycotinine from saliva;
  • Hydroxylated cholesterols in plasma by LCMS
  • Coenzyme Q10 in plasma by HPLC/ECD (oxidized, reduced and total Q10)
  • Creatinine in urine by AA
  • CRP in plasma by AA
  • DNA by HPLC
  • DNA methylation, global (5-methyl-dC/dG)
  • Fatty acids in plasma or RBC's by high-accuracy LCMS (myristic acid [MA, C14:0], stearic acid [SA, C18:0], palmitic acid [PA, C16:0], oleic acid [OA, C18:1], palmitoleic acid [PLA, C16:1], docosapentaenoic acid [DPA, C22:5 n-3], docosahexaenoic acid [DHA, C22:6 n-3], eicosapentaenoic acid [EPA, C20:5 n-3], Î±-linolenic acid [ALA, C18:3 n-3], eicosadienoic acid [EDA, C20:2 n-6], linoleic acid [LA, C18:2 n-6], arachidonic acid [AA, C20:4 n-6], Î±-eleostearic acid [αESA, C18:3]; stearic acid-d3 [SA-d3], linoleic acid-d4 [LA-d4], arachidonic acid-d8 [AA-d8], palmitoleic acid-d14 [PLA-d14], Eicosapentaenoic acid-d5 [EPA-d5], docosahexaenoic acid-d5 [DHA-d5], or any other fatty acid)
  • Folates in plasma by LC/MSMS
  • Glyphosate (Roundup), glufosinate and AMPA from urine by LCMS
  • Haptoglobin in plasma by AA
  • HDL-cholesterol in plasma by AA
  • Homocysteine, cysteine in plasma by LCMS
  • Isoflavonoids, flavonoids, lignans, anthocyanins or other groups of phenolic agents from plasma, urine, breast milk, foods by LC/MSMS
  • L-alanine, L-glutamic acid, L-glutamine, glucose-6-phosphate from liver tissue
  • Lipid panel in plasma by AA (Chol, HDLC, triglycerides)
  • Lipid-Phase Micronutrients in plasma or foods by HPLC/PDA/FD (12 carotenoids, 2 retinoids, 3 tocopherols)
  • Metabolomics by LCMS
  • Methylmalonic acid in plasma by LC/MSMS
  • Neurotransmitters in CSF or plasma/serum by HPLC/FD/ECD: norepinephrine, 5-hydroxyindole-3-acetic acid, homovanillic acid, kynurenic acid, L-kynurenine, tryptophane
  • Nicotine and metabolites from urine, saliva, hair, plants by LCMS (nicotine, nornicotine, anabasine, myosmine, cotinine, hydroxycotinine, NNN [N-nitrosonornicotine], NNK [4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone], NAB [N-nitrosoanabasine], and NAT [N-nitrosoanatabine])
  • Nitrogen, urea in urine by AA
  • Oxytocin from serum by LCMS
  • Parabens from urine by LCMS
  • Phenoxyacetic acids (2,4-D; 2,4-T) from urine by LCMS
  • Phospholipids (the most abundant arrays of phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, lysophosphatidylcholines, and phosphatidylinositols) from liver tissue
  • Phthalates in urine by LC/MSMS (phthalate, monoethyl phthalate, mono-n-butyl phthalate, mono-isobutyl phthalate, monobenzyl phthalate, mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-hydroxyhexyl) phthalate, mono(2-ethyl-5-oxohexyl) phthalate, mono(2-ethyl-5-carboxypentyl) phthalate)
  • Red blood cell preparation
  • Roundup (glyphosate) and aminomethyl phosphonic acid (AMPA) from urine by LCMS
  • Sucrose, fructose, glucose in urine by LC/MSMS
  • Total phenol levels in food, urine, plasma
  • Triclosan from urine by LCMS
  • Triglycerides in plasma by AA**
  • Trimethylamino-N-oxide (TMAO), choline, betaine, and carnithine from serum
  • Vanillin from e-cigarette juices by LCMS
  • Vitamin A and pro-vitamin A in plasma by HPLC/PDA
  • Vitamin B group in plasma by LC/MS (pyridoxic acid, pyridoxal phosphate; folate, 5-methyltetrahydrofolate; methylmalonic acid; homocysteine, cysteine; choline, betaine)
  • Vitamin C in plasma, food by HPLC/ECD or PDA
  • Vitamin D in plasma by LC/MSMS (vitamin D2, vitamin D3, 25-hydroxy-vitamin D2, and 25-hydroxy-vitamin D3)
  • Vitamin E in plasma by NP-HPLC/PDA/FD (all tocopherols)
  • Vitamin K by LC/MSMS
  • Vitamin and antioxidant panel (lipid soluble) from plasma by single HPLC/PDA/FD (25-hydroxy-vitamin D2 and 25-hydroxy-vitamin D3; alpha-, beta-, gamma-, and delta-tocopherol; all-trans and geometric isomers of lutein, zeaxanthin, alpha- and beta-cryptoxanthin, lycopene, and alpha- and beta-carotene; retinol and retinyl palmitate; ubiquinol-10 and ubiquinone-10)
  • Steroids in urine and plasma/serum by LC/MS (estriol [E3], 16-epiestriol [16epi-E3], 17-epiestriol [17epi-E3], 16-ketoestradiol [16keto-E2], 16-α-hydroxyestrone [16αOH-E1], 2-methoxyestrone [2MeO-E1], 3-methoxyestrone [3Me-OE1], 4-methoxyestrone [4MeO-E1], 2-methoxyestradiol [2MeO-E2], 4-methoxyestradiol [4MeO-E2], 2-hydroxyestrone [2OH-E1], 4-hydroxyestrone [4OH-E1], 2-hydroxyestradiol [2OH-E2], progesterone [P], testosterone [T]), corticoids (cortisol, cortisone, etc.) and other steroids
  • Any other assay based on clinical autoanalyzer technique (Cobas MiraPlus CC)

Enzyme immunoassays

Equipment use

  • HPLC/PDA systems (no supplies)
  • LC/MS system (no supplies)

Consultation

  • technical (staff)
  • professional (director)
  • lab experiments (total analysis including supplies)

All plasma assays can be done from serum.
All other ELISA assays can be performed upon request. Please contact Dr. Franke, This e-mail address is being protected from spambots. You need JavaScript enabled to view it. .

AA = clinical chemistry auto analyzer (Cobas MiraPlus CC)
ECD = electrochemical detection
FD = fluorescence detectionHPLC = high pressure liquid chromatography
HPLC = high pressure liquid chromatography
LC/MS = liquid chromatography mass spectrometry
LC/MSMS = liquid chromatography tandem mass spectrometry
NP = normal-phase
PDA = photo diode array detection
RBC = red blood cells

Resources and Instrumentation in this laboratory

  • 3 fully automated liquid chromatography mass spectrometry (LC/MS) systems: 2 orbitrap (model Q-Exactive, Thermo, Woburn MA) and 1 triple quadrupole instrument (model TSQ Ultra, Thermo), all equipped with electrospray or atmospheric pressure chemical ionization or atmospheric pressure photo ionization, all allowing positive and negative ion monitoring. LC models are all Accela (Thermo)
  • 3 fully automated high pressure liquid chromatography (HPLC) systems (model Surveyor, Thermo) and 2 ultra high pressure liquid chromatography systems (UHPLC; model Accela, Thermo, attached to the mass spectrometers above), all with quaternary pumps that can all be connected to above MS systems or/and to one of 5 available photo-diode array (PDA) detectors or to fluorescence (FL; model FD100, GTI/SpectroVision, Concord, MA) or electrochemical detectors (Coulochem III with 5021 dual cell or Coularray with 8 cells, ESA, Chelmsford, MA) devices
  • 2 nitrogen generators (Peak Scientific, Billerica, MA)
  • 1 post column derivatization system (Timberline Co., Boulder, CO)
  • For routine ELISA assays: 2 model 'VersaMax' microplate reader with 'Analyst AD' capabilities for UV/fluorescence readings (Molecular Devices, Sunnyvale, CA) and one Luminex200 instrument (Luminex Corpor., Austin, TX) for multiplexing immunoassays
  • 1 spectrophotometer (model BioSpec160I, Shimadzu Co., Columbia, MD)
  • 2 clinical autoanalyzers (Roche Cobas Mira Plus CC, Roche Diagnostics Inc., Indianapolis, IN)
  • 1 liquid handling robot (model Versa 100, Aurorabiomed, Vancouver, BC)
  • 2 rotating evaporators (BĂŒchi, Switzerland)
  • 2 centrifuges (IEC Co., Vermont Hill, IL),
  • 2 8ÂșC refrigerators
  • 1 -4ÂșC freezer
  • 1 -20ÂșC freezer
  • 2 -80ÂșC freezers (Fisher Scientific Co., VWR Scientific Co.)
  • 3 biological fume hood with laminar flow (Labconco, Kansas City, MO)
  • one 4 TB backup system for data storage and archiving

Other equipment this operation has access to includes a solid-phase extraction system, nitrogen gas evaporation system, rotary evaporation system, and a complete Millipore water filter system with ion exchange columns (all from Waters, Milford, MA). Additional shared equipment includes a Beckman L2-65B and a L8-70M ultracentrifuges (SW55 Ti, SW27, 70 Ti, SW41 rotors), Sorvall OTD-50 ultracentrifuge (TV865 rotor), a lyophilizer (Labconco), a Beckman LS 7500 and LS100 liquid scintillation counters, a Beckman Gamma 5500B counter, a model AS 160 speed-vac (Savant, Farmingdale, NY) for evaporation of small multiple samples, five ultra-low temperature freezers (Forma Scientif. Co., Queue Systems, Revco Scientific Co., Asheville, NC), a Coulter automatic cell counter, a fluorometer (Perkin-Elmer Co., Cupertino, CA), and several sterilizers including a high pressure vapor sterilizer.

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