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Microscopy and Imaging

The Microscopy and Imaging Shared Resource was established to fulfill a critical need for Cancer Center researchers who need to examine tissues and cells at the subcellular level; to count, isolate and measure cells; and to utilize non-invasive techniques to monitor tumorigenesis in mouse models. This capability is only possible through the use of state-of-the-art instrumentation. This shared resource has evolved from the previous Laboratory Instrumentation Shared Resource, and now has expanded imaging capabilities through recent acquisition of several important instruments.

This resource offers technical training to users, and maintains the complex instrumentation through annual maintenance services and routine calibrations. It is also responsible for upgrades, repairs, and acquisition of new equipment according to the needs of the Cancer Center researchers.

Currently the resource houses six major instruments and three microscopes for general fluorescence and brightfield use. It also offers access to a licensed MetaMorph software package for the processing and analysis of microscopy images.

Major imaging instrumentation

  • Leica TCS SP5 laser confocal microscope
  • Laser capture micro-dissection system
  • Olympus IX81 time-lapse system with environmental chamber
  • IVIS Lumina (Located in the Biosciences Building Vivarium)
  • Becton Dickinson Accuri C6 flow cytometer
  • Becton Dickinson LSRFortessa cell analyzer, with 12 colors (Located in the Department of Tropical Medicine, JABSOM, Biosciences Building)

General microscopy

  • Zeiss Axioplan with Coolsnap color camera
  • Olympus IX71 with Qimaging CCD monochrome camera
  • Nikon Diaphot inverted microscope

Services

  • Access to instruments and basic supplies
  • Training – Initial and refresher training; consultation, assisted sessions and troubleshooting, including consultation services with the Pathology Shared Resource for studies related to laser capture micro-dissection.

Equipment located within the Cancer Center labs is available 24 hours per day, seven days per week for trained Cancer Center members. Non-members can access the instruments during regular business hours, Monday through Friday, 9 to 6 p.m. Exceptions may be granted by the Scientific Manager. Assisted sessions are held only during regular business hours. Access to the IVIS Lumina instrument requires clearance from the Laboratory of Animal Services. Access to the LSRFortessa cell analyzer must be scheduled in advance with the Scientific Manager or the Research Coordinator.  


Faculty Director:

Patricia S. Lorenzo, PhD
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Scientific Manager:

Chris Farrar, PhD
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Research Coordinator:

Cynthia Rajani, PhD
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